Chamberlain Lab

Slide 1

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Confocal Microscopy is a common approach used to study the intracellular localisation and trafficking of proteins in either fixed or live cells. This technique is used widely in the group to investigate how S-acylation affects protein localisation and also to identify targeting signals present in DHHC enzymes. We employ deconvolution of image stacks (using Huygen’s software) as standard, and implement quantitative measurements of protein co-localisation. In addition, we use approaches such as Fluorescence Recovery After Photobleaching (FRAP) or inverse FRAP (iFRAP) to visualize protein localisation changes in real time.