Chamberlain Lab

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Peptide array based screening reveals a large number of proteins interacting with the ankyrin repeat domain of the zDHHC17 S-acyltransferase.

Lemonidis K, MacLeod R, Baillie GS, Chamberlain LH.

J Biol Chem. 2017 Sep 7. pii: jbc.M117.799650. doi: 10.1074/jbc.M117.799650. [Epub ahead of print]

PMID: 28882895 Free Article

The C-terminal domain of zDHHC2 contains distinct sorting signals that regulate intracellular localisation in neurons and neuroendocrine cells.

Salaun C, Ritchie L, Greaves J, Bushell TJ, Chamberlain LH.

Mol Cell Neurosci. 2017 Jul 30. pii: S1044-7431(17)30126-4. doi: 10.1016/j.mcn.2017.07.007. [Epub ahead of print]

PMID: 28768144 Free Article

Substrate selectivity in the zDHHC family of S-acyltransferases.

Lemonidis K, Salaun C, Kouskou M, Diez-Ardanuy C, Chamberlain LH, Greaves J.

Biochem Soc Trans. 2017 Jun 15;45(3):751-758. doi: 10.1042/BST20160309. Review.

PMID: 28620036

Molecular basis of fatty acid selectivity in the zDHHC family of S-acyltransferases revealed by click chemistry.

Greaves J, Munro KR, Davidson SC, Riviere M, Wojno J, Smith TK, Tomkinson NC, Chamberlain LH.

Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201612254. doi: 10.1073/pnas.1612254114. [Epub ahead of print]

PMID: 28167757 Free Article

A cluster of palmitoylated cysteines are essential for aggregation of cysteine-string protein mutants that cause neuronal ceroid lipofuscinosis.

Diez-Ardanuy C, Greaves J, Munro KR, Tomkinson NC, Chamberlain LH.

Sci Rep. 2017 Dec;7(1):10. doi: 10.1038/s41598-017-00036-8.

PMID: 28127059 Free Article

Obesogenic and Diabetogenic Effects of High-Calorie Nutrition Require Adipocyte BK Channels.

Illison J, Tian L, McClafferty H, Werno M, Chamberlain LH, Leiss V, Sassmann A, Offermanns S, Ruth P, Shipston MJ, Lukowski R.

Diabetes. 2016 Dec;65(12):3621-3635.

PMID: 27605626

S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines.

Werno MW, Chamberlain LH.

Sci Rep. 2015 Jul 22;5:12413. doi: 10.1038/srep12413.

PMID: 26198666 Free Article

Identification of a novel sequence motif recognised by the ankyrin-repeat domain of zDHHC17/13 S-acyl-transferases.

Lemonidis K, Sanchez-Perez MC, Chamberlain LH.

J Biol Chem. 2015 Jul 21. pii: jbc.M115.657668. [Epub ahead of print]

PMID: 26198635 Free Article

The zDHHC family of S-acyltransferases.

Lemonidis K, Werno MW, Greaves J, Diez-Ardanuy C, Sanchez-Perez MC, Salaun C, Thomson DM,Chamberlain LH.

Biochem Soc Trans. 2015 Apr;43(2):217-21. doi: 10.1042/BST20140270.

PMID: 25849920

The physiology of protein S-acylation.

Chamberlain LH, Shipston MJ.

Physiol Rev. 2015 Apr;95(2):341-76. doi: 10.1152/physrev.00032.2014. Review.

PMID: 25834228

A molecular toggle after exocytosis sequesters the presynaptic syntaxin1a molecules involved in prior vesicle fusion.

Kavanagh DM, Smyth AM, Martin KJ, Dun A, Brown ER, Gordon S, Smillie KJ,Chamberlain LH, Wilson RS, Yang L, Lu W, Cousin MA, Rickman C, Duncan RR.

Nat Commun. 2014 Dec 17;5:5774. doi: 10.1038/ncomms6774.

PMID: 25517944 Free PMC Article

The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.

Lemonidis K, Gorleku OA, Sanchez-Perez MC, Grefen C, Chamberlain LH.

Mol Biol Cell. 2014 Dec 1;25(24):3870-83. doi: 10.1091/mbc.E14-06-1169. Epub 2014 Sep 24.

PMID: 25253725 Free PMC Article

New links between S-acylation and cancer.

Greaves J, Chamberlain LH.

J Pathol. 2014 May;233(1):4-6. doi: 10.1002/path.4339.

PMID: 24615251

The cytosolic N-terminus of CD317/tetherin is a membrane microdomain exclusion motif.

Billcliff PG, Gorleku OA, Chamberlain LH, Banting G.

Biol Open. 2013 Oct 15;2(11):1253-63. doi: 10.1242/bio.20135793. eCollection 2013.

PMID: 24244863 Free PMC Article

Evolving views on protein palmitoylation (Comment on DOI 10.1002/bies.201300076).

Chamberlain LH.

Bioessays. 2013 Nov;35(11):928. doi: 10.1002/bies.201300121. Epub 2013 Sep 10. No abstract available.

PMID: 24019033

Palmitoylation and the trafficking of peripheral membrane proteins.

Chamberlain LH, Lemonidis K, Sanchez-Perez M, Werno MW, Gorleku OA, Greaves J.

Biochem Soc Trans. 2013 Feb 1;41(1):62-6. doi: 10.1042/BST20120243.

PMID: 23356259

Palmitoylation-induced aggregation of cysteine-string protein mutants that cause neuronal ceroid lipofuscinosis.

Greaves J, Lemonidis K, Gorleku OA, Cruchaga C, Grefen C, Chamberlain LH.

J Biol Chem. 2012 Oct 26;287(44):37330-9. doi: 10.1074/jbc.M112.389098. Epub 2012 Aug 19.

PMID: 22902780   Free PMC Article

Endoplasmic reticulum localization of DHHC palmitoyltransferases mediated by lysine-based sorting signals.

Gorleku OA, Barns AM, Prescott GR, Greaves J, Chamberlain LH.

J Biol Chem. 2011 Nov 11;286(45):39573-84. doi: 10.1074/jbc.M111.272369. Epub 2011 Sep 18.

PMID: 21926431   Free PMC Article

Regional and developmental brain expression patterns of SNAP25 splice variants.

Prescott GR, Chamberlain LH.

BMC Neurosci. 2011 Apr 28;12:35. doi: 10.1186/1471-2202-12-35.

PMID: 21526988  Free PMC Article

The palmitoyl transferase DHHC2 targets a dynamic membrane cycling pathway: regulation by a C-terminal domain.

Greaves J, Carmichael JA, Chamberlain LH.

Mol Biol Cell. 2011 Jun 1;22(11):1887-95. doi: 10.1091/mbc.E10-11-0924. Epub 2011 Apr 6.

PMID: 21471008 Free PMC Article

Differential palmitoylation regulates intracellular patterning of SNAP25.

Greaves J, Chamberlain LH.

J Cell Sci. 2011 Apr 15;124(Pt 8):1351-60. doi: 10.1242/jcs.079095. Epub 2011 Mar 23.

PMID: 21429935 Free PMC Article

DHHC palmitoyl transferases: substrate interactions and (patho)physiology.

Greaves J, Chamberlain LH.

Trends Biochem Sci. 2011 May;36(5):245-53. doi: 10.1016/j.tibs.2011.01.003. Epub 2011 Mar 8. Review.

PMID: 21388813

The intracellular dynamic of protein palmitoylation.

Salaun C, Greaves J, Chamberlain LH.

J Cell Biol. 2010 Dec 27;191(7):1229-38. doi: 10.1083/jcb.201008160. Review.

PMID: 21187327 Free PMC Article

Palmitoylation of the SNAP25 protein family: specificity and regulation by DHHC palmitoyl transferases.

Greaves J, Gorleku OA, Salaun C, Chamberlain LH.

J Biol Chem. 2010 Aug 6;285(32):24629-38. doi: 10.1074/jbc.M110.119289. Epub 2010 Jun 2.

PMID: 20519516 Free PMC Article

Palmitoylation and testis-enriched expression of the cysteine-string protein beta isoform.

Gorleku OA, Chamberlain LH.

Biochemistry. 2010 Jun 29;49(25):5308-13. doi: 10.1021/bi100550h.

PMID: 20499929

S-acylation by the DHHC protein family.

Greaves J, Chamberlain LH.

Biochem Soc Trans. 2010 Apr;38(2):522-4. doi: 10.1042/BST0380522. Review.

PMID: 20298214

t-SNARE protein conformations patterned by the lipid microenvironment.

Rickman C, Medine CN, Dun AR, Moulton DJ, Mandula O, Halemani ND, Rizzoli SO, Chamberlain LH, Duncan RR.

J Biol Chem. 2010 Apr 30;285(18):13535-41. doi: 10.1074/jbc.M109.091058. Epub 2010 Jan 21.

PMID: 20093362 Free PMC Article

Regulation of SNAP-25 trafficking and function by palmitoylation.

Greaves J, Prescott GR, Gorleku OA, Chamberlain LH.

Biochem Soc Trans. 2010 Feb;38(Pt 1):163-6. doi: 10.1042/BST0380163. Review.

PMID: 20074052

Palmitoylation of the synaptic vesicle fusion machinery.

Prescott GR, Gorleku OA, Greaves J, Chamberlain LH.

J Neurochem. 2009 Aug;110(4):1135-49. doi: 10.1111/j.1471-4159.2009.06205.x. Epub 2009 Jun 5. Review.

PMID: 19508429

Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity.

Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I.

J Immunol. 2009 Jun 15;182(12):8026-36. doi: 10.4049/jimmunol.0900107.

PMID: 19494328 Free Article

The neuropathic potential of anti-GM1 autoantibodies is regulated by the local glycolipid environment in mice.

Greenshields KN, Halstead SK, Zitman FM, Rinaldi S, Brennan KM, O'Leary C,Chamberlain LH, Easton A, Roxburgh J, Pediani J, Furukawa K, Furukawa K, Goodyear CS, Plomp JJ, Willison HJ.

J Clin Invest. 2009 Mar;119(3):595-610. doi: 10.1172/JCI37338. Epub 2009 Feb 16.

PMID:19221437 Free PMC Article

The hydrophobic cysteine-rich domain of SNAP25 couples with downstream residues to mediate membrane interactions and recognition by DHHC palmitoyl transferases.

Greaves J, Prescott GR, Fukata Y, Fukata M, Salaun C, Chamberlain LH.

Mol Biol Cell. 2009 Mar;20(6):1845-54. doi: 10.1091/mbc.E08-09-0944. Epub 2009 Jan 21.

PMID: 19158383 Free PMC Article

The fat controller: roles of palmitoylation in intracellular protein trafficking and targeting to membrane microdomains (Review).

Greaves J, Prescott GR, Gorleku OA, Chamberlain LH.

Mol Membr Biol. 2009 Jan;26(1):67-79. doi: 10.1080/09687680802620351. Epub 2008 Dec 29. Review.

PMID: 19115144

Palmitoylation gates phosphorylation-dependent regulation of BK potassium channels.

Tian L, Jeffries O, McClafferty H, Molyvdas A, Rowe IC, Saleem F, Chen L, Greaves J, Chamberlain LH, Knaus HG, Ruth P, Shipston MJ.

Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):21006-11. doi: 10.1073/pnas.0806700106. Epub 2008 Dec 19.

PMID:19098106 Free PMC Article

Palmitoylation and membrane interactions of the neuroprotective chaperone cysteine-string protein.

Greaves J, Salaun C, Fukata Y, Fukata M, Chamberlain LH.

J Biol Chem. 2008 Sep 5;283(36):25014-26. doi: 10.1074/jbc.M802140200. Epub 2008 Jul 2.

PMID: 18596047   Free PMC Article

Munc18-1 prevents the formation of ectopic SNARE complexes in living cells.

Medine CN, Rickman C, Chamberlain LH, Duncan RR.

J Cell Sci. 2007 Dec 15;120(Pt 24):4407-15.

PMID: 18057031 Free Article

Palmitoylation-dependent protein sorting.

Greaves J, Chamberlain LH.

J Cell Biol. 2007 Jan 29;176(3):249-54. Epub 2007 Jan 22. Review.

PMID:17242068  Free PMC Article

Dual role of the cysteine-string domain in membrane binding and palmitoylation-dependent sorting of the molecular chaperone cysteine-string protein.

Greaves J, Chamberlain LH.

Mol Biol Cell. 2006 Nov;17(11):4748-59. Epub 2006 Aug 30.

PMID: 16943324 Free PMC Article

Lipid raft association of SNARE proteins regulates exocytosis in PC12 cells.

Salaün C, Gould GW, Chamberlain LH.

J Biol Chem. 2005 May 20;280(20):19449-53. Epub 2005 Mar 15.

PMID: 15769746 Free PMC Article

Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes.

Ewart MA, Clarke M, Kane S, Chamberlain LH, Gould GW.

J Biol Chem. 2005 Feb 4;280(5):3812-6. Epub 2004 Nov 17.

PMID: 15550383 Free Article

The SNARE proteins SNAP-25 and SNAP-23 display different affinities for lipid rafts in PC12 cells. Regulation by distinct cysteine-rich domains.

Salaün C, Gould GW, Chamberlain LH.

J Biol Chem. 2005 Jan 14;280(2):1236-40. Epub 2004 Nov 12.

PMID: 15542596 Free PMC Article

Plasma membrane targeting of exocytic SNARE proteins.

Salaün C, James DJ, Greaves J, Chamberlain LH.

Biochim Biophys Acta. 2004 Aug 23;1693(2):81-9. Review.

PMID: 15313010

Lipid rafts and the regulation of exocytosis.

Salaün C, James DJ, Chamberlain LH.

Traffic. 2004 Apr;5(4):255-64. Review.

PMID: 15030567 Free PMC Article

Neomycin prevents the wortmannin inhibition of insulin-stimulated Glut4 translocation and glucose transport in 3T3-L1 adipocytes.

James DJ, Salaün C, Brandie FM, Connell JM, Chamberlain LH.

J Biol Chem. 2004 May 14;279(20):20567-70. Epub 2004 Mar 15.

PMID: 15024008 Free Article

Detergents as tools for the purification and classification of lipid rafts.

Chamberlain LH.

FEBS Lett. 2004 Feb 13;559(1-3):1-5. Review.

PMID: 14986659

Syntaxin 6 regulates Glut4 trafficking in 3T3-L1 adipocytes.

Perera HK, Clarke M, Morris NJ, Hong W, Chamberlain LH, Gould GW.

Mol Biol Cell. 2003 Jul;14(7):2946-58. Epub 2003 Apr 4.

PMID: 12857877 Free PMC Article

The vesicle- and target-SNARE proteins that mediate Glut4 vesicle fusion are localized in detergent-insoluble lipid rafts present on distinct intracellular membranes.

Chamberlain LH, Gould GW.

J Biol Chem. 2002 Dec 20;277(51):49750-4. Epub 2002 Oct 9.

PMID: 12376543 Free Article

Inhibition of isoprenoid biosynthesis causes insulin resistance in 3T3-L1 adipocytes.

Chamberlain LH.

FEBS Lett. 2001 Nov 2;507(3):357-61.

PMID: 11696371

Phosphorylation of cysteine string protein by protein kinase A. Implications for the modulation of exocytosis.

Evans GJ, Wilkinson MC, Graham ME, Turner KM, Chamberlain LH, Burgoyne RD, Morgan A.

J Biol Chem. 2001 Dec 21;276(51):47877-85. Epub 2001 Oct 16.

PMID: 11604405 Free Article

SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.

Chamberlain LH, Burgoyne RD, Gould GW.

Proc Natl Acad Sci U S A. 2001 May 8;98(10):5619-24. Epub 2001 May 1.

PMID: 11331757 Free PMC Article

The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4.

Chamberlain LH, Graham ME, Kane S, Jackson JL, Maier VH, Burgoyne RD, Gould GW.

J Cell Sci. 2001 Jan;114(Pt 2):445-55.

PMID: 11148145 Free Article

Cysteine-string protein: the chaperone at the synapse.

Chamberlain LH, Burgoyne RD.

J Neurochem. 2000 May;74(5):1781-9. Review.

PMID: 10800920

Quantification of SNARE protein levels in 3T3-L1 adipocytes: implications for insulin-stimulated glucose transport.

Hickson GR, Chamberlain LH, Maier VH, Gould GW.

Biochem Biophys Res Commun. 2000 Apr 21;270(3):841-5.

PMID: 10772913

Mutational analysis of cysteine-string protein function in insulin exocytosis.

Zhang H, Kelley WL, Chamberlain LH, Burgoyne RD, Lang J.

J Cell Sci. 1999 May;112 ( Pt 9):1345-51.

PMID: 10194413 Free Article

Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes.

Zhang H, Kelley WL, Chamberlain LH, Burgoyne RD, Wollheim CB, Lang J.

FEBS Lett. 1998 Oct 23;437(3):267-72.

PMID: 9824305

The cysteine-string domain of the secretory vesicle cysteine-string protein is required for membrane targeting.

Chamberlain LH, Burgoyne RD.

Biochem J. 1998 Oct 15;335 ( Pt 2):205-9.

PMID: 9761715 Free PMC Article

Cysteine string protein functions directly in regulated exocytosis.

Chamberlain LH, Burgoyne RD.

Mol Biol Cell. 1998 Aug;9(8):2259-67.

PMID: 9693380 Free PMC Article

The molecular chaperone function of the secretory vesicle cysteine string proteins.

Chamberlain LH, Burgoyne RD.

J Biol Chem. 1997 Dec 12;272(50):31420-6.

PMID: 9395474 Free Article

Activation of the ATPase activity of heat-shock proteins Hsc70/Hsp70 by cysteine-string protein.

Chamberlain LH, Burgoyne RD.

Biochem J. 1997 Mar 15;322 ( Pt 3):853-8.

PMID: 9148760 Free PMC Article

Cysteine string proteins are associated with chromaffin granules.

Chamberlain LH, Henry J, Burgoyne RD.

J Biol Chem. 1996 Aug 9;271(32):19514-7.

PMID: 8702643Free Article

SNAPs and SNAREs in exocytosis in chromaffin cells.

Burgoyne RD, Morgan A, Barnard RJ, Chamberlain LH, Glenn DE, Kibble AV.

Biochem Soc Trans. 1996 Aug;24(3):653-7. Review. No abstract available.

PMID: 8878820

Identification of a novel cysteine string protein variant and expression of cysteine string proteins in non-neuronal cells.

Chamberlain LH, Burgoyne RD.

J Biol Chem. 1996 Mar 29;271(13):7320-3.

PMID: 8631751 Free Article

Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis.

Chamberlain LH, Roth D, Morgan A, Burgoyne RD.

J Cell Biol. 1995 Sep;130(5):1063-70.

PMID: 7657692 Free PMC Article