Chamberlain Lab

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We employ a wide range of techniques to study the mechanisms and functional outcomes of S-acylation. These include standard molecular and biochemical techniques, such as DNA cloning and mutagenesis, recombinant protein expression and purification, cell culture and transfection, and antibody capture and detection systems.. In addition to these standard techniques, we employ confocal microscopy approaches including live cell analyses of protein trafficking (FRAP, iFRAP, antibody uptake) and quantitative co-localisation analyses on deconvolved whole cell image stacks, Furthermore, we have implemented several high-sensitivity assays of protein S-acylation, such as Acyl-RAC and click chemistry. In this section, we will describe the principles of some of these newer techniques adopted by the group.  If you would like to know more about our work or specific techniques, please contact us.